Thermo Fisher Scientific GNAS Monoclonal Antibody (OTI7A4), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:500

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).

Target Information

Mutations in the GNAS gene are associated with several disorders, including:

• Pseudohypoparathyroidism type 1a and 1b
• Albright hereditary osteodystrophy
• Pseudopseudohypoparathyroidism
• McCune-Albright syndrome
• Progressive osseous heteroplasia
• Polyostotic fibrous dysplasia of bone
• Certain pituitary tumors

GNAS exhibits a complex imprinted expression pattern, producing multiple alternatively spliced transcripts with distinct 5′ exons. These exons are located within differentially methylated regions typical of imprinted genes. The gene also contains oppositely expressed promoter regions in close proximity (~14 kb), and one alternate exon introduces a frameshift leading to a structurally distinct isoform. An antisense transcript may regulate imprinting in this region.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.