Thermo Fisher Scientific HLA-DR Monoclonal Antibody (LN3), eFluor 450, eBioscience
휴먼 HLA-DR 항원을 인식하는 LN3 클론 단일클론 항체로, eFluor 450 형광표지되어 Flow Cytometry에 최적화됨. 인간 APC 표면의 MHC class II 검출용. 405 nm Violet 레이저에 적합하며, 고순도 친화 크로마토그래피 정제.
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Thermo Fisher Scientific HLA-DR Monoclonal Antibody (LN3), eFluor 450, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 4 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test | View 20 publications |
| ELISA | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 450 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | LN3 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10718248 |
Available Formats
- Unconjugated
- Alexa Fluor 660
- Alexa Fluor 700
- Alexa Fluor Plus 488
- Alexa Fluor Plus 647
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 480 / 605 / 711 / 786
- eFluor 506
- FITC
- NovaFluor Blue, Violet, Yellow series
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright series (436, 600, 645, 702, 780)
- Request custom conjugation available
Product Specific Information
The LN3 monoclonal antibody reacts with human MHC class II molecule HLA-DR, expressed on antigen presenting cells such as B cells, monocytes, macrophages, dendritic cells, and activated T cells.
HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits, essential for peptide presentation to CD4⁺ T lymphocytes.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated and validated on normal human peripheral blood cells.
Recommended use: 5 µL (0.125 µg) per test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test. - Fluorochrome Information: eFluor® 450 emits at 445 nm, excited at 405 nm Violet laser.
Ensure instrument compatibility with this fluorochrome. - Filtration: 0.2 µm post-manufacturing filtered.
Target Information
HLA-DR is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and a 27 kDa beta chain (DRB).
The alpha chain gene (DRA) has 5 exons, encoding leader peptide, extracellular domains, transmembrane domain, and cytoplasmic tail.
DRA acts as the sole alpha chain for DRB1, DRB3, DRB4, and DRB5, while DRB1 alleles define peptide binding specificity.
HLA-DR is expressed mainly on antigen presenting cells (B lymphocytes, monocytes, macrophages, thymic epithelial cells, activated T lymphocytes).
MHC class II molecules (DR, DQ, DP) present processed peptides to T-helper cells, initiating immune responses.
HLA and MHC antibodies are crucial in Immunopeptidomics, aiding neoantigen identification and characterization via LC-MS/MS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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