Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (MIH4), eFluor 450, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 645
• Request custom conjugation

Product Specific Information

The MIH4 monoclonal antibody reacts with human PD-1 (programmed death-1), a 55 kDa member of the immunoglobulin superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes.

Two ligands of PD-1, PD-L1 (B7-H1) and PD-L2 (B7-DC), have overlapping functions and are expressed on normal tissues and activated antigen-presenting cells. The MIH4 antibody recognizes a different epitope than clone J105.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested by flow cytometry using stimulated normal human peripheral blood cells.
Recommended use: 5 µL (0.5 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

eFluor™ 450 is a replacement for Pacific Blue™, excited at 405 nm and emitting at 445 nm. Ensure the instrument can detect this fluorochrome.

Excitation: 405 nm
Emission: 445 nm
Laser: Violet Laser

Target Information

T-cell activation is regulated by inhibitory receptors such as PD-1 (PDCD-1), which binds to ligands PD-L1 and PD-L2 on antigen-presenting cells. This binding inhibits immune activation in the absence of danger signals. PD-1-deficient mice exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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