Thermo Fisher Scientific HSPB8 Monoclonal Antibody (3C12-H11), PE
HSPB8 단백질 검출용 마우스 단클론 항체로, PE 형광 표지된 제품입니다. Western blot, IHC, ICC/IF, ELISA 등 다양한 응용에 사용 가능합니다. 인간, 마우스, 랫트 반응성. 단백질 G 정제, 보존제 없음, 4°C 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3C12-H11 |
| Immunogen | His-tagged human recombinant HSP22 |
| Conjugate | PE |
| Excitation/Emission Max | 565/576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 47.35 mM phosphate / 2.15 mM MES, pH 7.4, with 1.033 mM EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931710 |
Additional Formats
- Unconjugated (MA5-27706)
- APC (MA5-45253)
- FITC (MA5-45254)
- PerCP (MA5-45255)
- Request custom conjugation available
Product Specific Information
1 µg/mL of MA5-45256 was sufficient for detection of HSP22 in 20 µg of whole rat tissue extract by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 22 kDa. Detects endogenous and exogenous HSP22 in monomeric, dimeric, and tetrameric forms in WB. Does not cross-react with alpha crystallin.
Target Information
The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. Expression is induced by estrogen in estrogen receptor-positive breast cancer cells. This protein functions as a chaperone in association with Bag3, a stimulator of macroautophagy, and is involved in regulation of cell proliferation, apoptosis, and carcinogenesis. Mutations in this gene have been associated with neuromuscular diseases, including Charcot-Marie-Tooth disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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