Thermo Fisher Scientific PCLO Monoclonal Antibody (6H9-B6), APC
PCLO 단백질을 인식하는 Thermo Fisher의 단클론 항체로, Human/Mouse/Rat 시료에 반응합니다. APC 형광 표지로 유세포분석 및 면역형광 실험에 적합하며, WB, ICC/IF, ELISA에 사용 가능합니다. 단백질 G 정제, 보존제 없음, 4°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6H9-B6 |
| Immunogen | Full length recombinant protein (GenBank ID: BC001304.1) |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931711 |
Additional Formats Available
- Unconjugated (MA5-27629)
- FITC (MA5-45258)
- PE (MA5-45260)
- PerCP (MA5-45259)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45257 was sufficient for detection of Piccolo in 20 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat IgG:HRP as the secondary antibody. Detects approximately 550 kDa. No cross-reactivity to Bassoon.
Target Information
The PCLO gene encodes a protein that is part of the presynaptic cytoskeletal matrix, involved in establishing active synaptic zones and synaptic vesicle trafficking. Variations in this gene have been associated with bipolar disorder and major depressive disorder. Two transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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