Thermo Fisher Scientific HIF-1 alpha Monoclonal Antibody (ESEE122), FITC
HIF-1 alpha 단백질을 인식하는 FITC 결합 단클론 항체로, Western blot, IHC, ICC/IF, ELISA에 적합합니다. 다양한 종(Bovine, Human, Mouse, Rat)에 반응하며, hypoxia 관련 연구에 활용됩니다. 형광 분석용으로 498/517 nm 파장 특성을 가집니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ESEE122 |
| Immunogen | Recombinant fragment corresponding to amino acids 329–530 of HIF1A |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.09 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931705 |
Additional Formats
- APC (Cat. No. MA5-45250)
- PerCP (Cat. No. MA5-45252)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45251 was sufficient for detection of HIF1 alpha in 20 µg of CoCl₂-induced HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 116 kDa. Specific for HIF1Alpha.
Target Information
HIF1-alpha (HIF1A) is a subunit of HIF1, a transcription factor found in mammalian cells under hypoxic conditions. HIF-1 is a heterodimer consisting of alpha and beta subunits, both part of the basic-helix-loop-helix PAS family of transcription factors. It regulates adaptive responses to hypoxia by activating transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, and VEGF.
HIF1-alpha influences hypoxia-mediated apoptosis, cell proliferation, and tumor angiogenesis. It interacts with p53 and participates in p300/CBP-HIF complexes that regulate hypoxia-responsive genes. Hypoxia contributes to diseases such as ischemia, cancer, pulmonary hypertension, and chronic obstructive pulmonary disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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