Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MP6-XT22), PE, eBioscience

Applications

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 561
• Alexa Fluor 660
• APC
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 786
• eFluor 450
• FITC
• Functional Grade
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells, and CD4(+) T cells. TNF alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. It forms dimers and trimers and also exists as a 26 kDa membrane-bound form.

Applications Reported:
This antibody is reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested:
Tested by intracellular flow cytometric analysis of stimulated splenocytes.
Use at 5 µL (0.125 µg) per test (100 µL final volume).
Cell number can range from 10⁵ to 10⁸ cells/test.
Excitation: 488–561 nm; Emission: 578 nm; Laser: Blue, Green, Yellow-Green.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine belonging to the TNF superfamily. It is mainly secreted by macrophages and binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha regulates immune cells, proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. It exists as multimers (dimers, trimers, pentamers) and shows a single 17 kDa band under non-reducing SDS-PAGE. Knockout studies in mice indicate neuroprotective functions and tumor necrosis activity. TNF alpha plays roles in immune responses to infections and tumor cytotoxicity, and is implicated in autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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