Thermo Fisher Scientific GM-CSF Monoclonal Antibody (MP1-22E9), PE, eBioscience

Thermo Fisher Scientific GM-CSF Monoclonal Antibody (MP1-22E9), PE, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular Staining)
• Tested Dilution: ≤ 0.25 µg/test
  (A test is defined as the amount of antibody that will stain a cell sample in 100 µL final volume. Cell number: 10⁵–10⁸ cells/test.)

Publications

• View 21 publications

Product Specifications

Additional Formats

• Unconjugated
• APC
• FITC
• Functional Grade
• PE-Cyanine7
• Custom conjugation available

Product Specific Information

The MP1-22E9 antibody reacts with mouse granulocyte/macrophage colony-stimulating factor (GM-CSF).
It is a neutralizing antibody that recognizes GM-CSF, a 14 kDa factor mainly produced by activated T cells and macrophages.
Other cell types such as endothelium and fibroblasts also secrete GM-CSF in response to TNF-α, IL-2, IL-1, and IFN-γ.
GM-CSF promotes the growth of macrophages, granulocytes, and dendritic cells, existing in both membrane-bound and extracellular matrix-associated forms.
Non-glycosylated GM-CSF retains biological activity.

Applications Reported: Intracellular flow cytometric analysis
Applications Tested: Intracellular staining and flow cytometric analysis of cultured mouse splenocytes
Recommended Excitation/Emission: 488–561 nm / 578 nm
Compatible Lasers: Blue, Green, Yellow-Green

Target Information

The GM-CSF gene encodes a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes.
The active form exists as a homodimer in the extracellular space.
Located on chromosome region 5q31, this gene cluster is associated with interstitial deletions in 5q- syndrome and acute myelogenous leukemia.
Other genes in the cluster include IL-4, IL-5, and IL-13.
The gene is involved in promoting tissue inflammation.
Elevated levels of GM-CSF have been observed in SARS-CoV-2 patients with acute respiratory distress syndrome.
Mice lacking this gene or its receptor develop pulmonary alveolar proteinosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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