Thermo Fisher Scientific EBI3 (IL-27 subunit) Monoclonal Antibody (ebic6), PE, eBioscience
인간 EBI3(IL-27 서브유닛)을 인식하는 ebic6 단클론 항체로, PE 형광체가 결합된 형태입니다. Flow Cytometry용으로 최적화되어 있으며, IL-27 및 IL-35 관련 면역조절 연구에 적합합니다. 4°C 암조건에서 보관하며, 동결 금지.
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Thermo Fisher Scientific EBI3 (IL-27 subunit) Monoclonal Antibody (ebic6), PE, eBioscience
Applications
- Flow Cytometry (intracellular staining)
Tested Dilution: 5 µL (0.03 µg)/test
Publications: View 3 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ebic6 |
| Immunogen | Human IL-27 recombinant protein |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10718237 |
Available Formats
Product Specific Information
This ebic6 monoclonal antibody reacts with human Epstein-Barr Virus Induced Gene 3 (EBI3).
EBI3 can be expressed and secreted as monomers or homodimers and was first identified in virally infected B cells. It is also secreted by trophoblast cells, potentially contributing to immune tolerance during pregnancy.
EBI3 forms heterodimers with:
- p28 (IL-30) → forming IL-27, influencing Th1 differentiation and suppressing Th2/Th17 responses.
- p35 (IL-12 subunit) → forming IL-35, produced by regulatory T cells with immunosuppressive functions.
ELISA data from human dendritic cells suggest that EBI3 detected by flow cytometry is largely dimerized with p28 (IL-27). Testing with ebic6 for IL-35 is ongoing.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated and validated using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824) and BestProtocols® (Protocol A).
Recommended Use: 5 µL (0.03 µg) per test (100 µL final volume; 10⁵–10⁸ cells/test).
Excitation: 488–561 nm; Emission: 578 nm; Laser: Blue, Green, Yellow-Green.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
EBI3 (Epstein-Barr Virus Induced-3) is a secreted glycoprotein that regulates Th2-type immune responses and tissue inflammation.
It dimerizes with IL-12 family subunits (p28 and p35) to form IL-27 and IL-35, respectively.
EBI3 expression is controlled by TLR signaling via MyD88 and NF-κB and plays roles in:
- Suppressing IL-17 and enhancing IL-10 production
- Regulating allergic airway inflammation
- Controlling tumor metastasis through CD8+ T cells
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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