Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MAb11), PE, eBioscience
인간 TNF-α를 인식하는 MAb11 단클론 항체로, PE 형광체가 결합되어 있습니다. 세포내 염색 및 유세포 분석에 최적화되어 있으며, 중화능을 가진 항체입니다. 488–561 nm 여기, 578 nm 방출 특성을 가지며, 연구용으로만 사용됩니다.
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Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MAb11), PE, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test |
| Miscellaneous PubMed (Misc) | - |
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MAb11 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10668834 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- Biotin
- Brilliant UV 661
- Brilliant UV 737
- Brilliant Violet 421
- Brilliant Violet 650
- eFluor 450
- FITC
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Request custom conjugation
Product Specific Information
The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells, and CD4+ T cells. TNF alpha has cytolytic activity against various tumor cells and plays a key role in immune regulation. It forms dimers and trimers and also exists as a 26 kDa membrane-bound form. MAb11 is a neutralizing antibody.
Applications Reported:
Used for intracellular staining followed by flow cytometric analysis.
Applications Tested:
Validated by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and the BestProtocols® Protocol A.
MAb11 antibody is pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. Recommended use is 5 µL (0.25 µg) per test in a final volume of 100 µL. Cell number can range from 10⁵ to 10⁸ cells/test.
Excitation / Emission / Laser:
488–561 nm excitation; 578 nm emission; compatible with Blue, Green, and Yellow-Green lasers.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
TNF alpha is a multifunctional proinflammatory cytokine belonging to the tumor necrosis factor (TNF) superfamily. It is mainly secreted by macrophages and binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha regulates immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. It exists as multimers and shows a single 17 kDa band under non-reducing SDS-PAGE conditions.
Knockout studies suggest its neuroprotective function, and TNF alpha induces tumor necrosis when injected into tumor-bearing mice. It is involved in immune responses to infections and tumor cell cytolysis, synergistic with interferon-gamma. TNF alpha is implicated in autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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