Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MP6-XT22), PE, eBioscience
TNF alpha를 인식하는 Mouse 반응성 단클론 항체로, PE 형광 표지되어 Flow Cytometry 분석에 적합합니다. 0.25 µg/test 농도로 사용 가능하며, 565/576 nm의 Excitation/Emission 특성을 가집니다. 면역조절 및 세포사멸 연구에 활용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 123 publications |
| Functional Assay | - | View 1 publication |
| In Situ Hybridization (ISH) | - | View 1 publication |
| Miscellaneous PubMed | - | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MP6-XT22 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466198 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 561
- Alexa Fluor 660
- APC
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 605
- Brilliant Violet 650
- Brilliant Violet 786
- eFluor 450
- FITC
- Functional Grade
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
Description:
The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells, and CD4(+) T cells. TNF alpha exhibits cytolytic activity against various tumor cells and plays an important role in immune regulation. It can form dimers and trimers and also exists as a 26 kDa membrane-bound form.
Applications Reported:
Used for intracellular staining followed by flow cytometric analysis.
Applications Tested:
Validated for intracellular flow cytometric analysis at ≤0.25 µg/test (100 µL final volume). Optimal titration recommended.
Excitation: 488–561 nm; Emission: 578 nm; Laser: Blue, Green, Yellow-Green.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
TNF alpha is a multifunctional proinflammatory cytokine belonging to the TNF superfamily. It is mainly secreted by macrophages and binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha regulates immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Exists as multimers and shows a 17 kDa band under non-reducing SDS-PAGE. Knockout mouse studies suggest neuroprotective functions and tumor necrosis induction. TNF alpha plays roles in immune responses to bacterial, viral, parasitic, and fungal infections, and is implicated in autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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