Thermo Fisher Scientific GLUT3 Polyclonal Antibody
Rabbit polyclonal antibody targeting human GLUT3 for WB, IHC, and ELISA applications. Recommended antigen retrieval using citrate buffer. Detects GLUT3 expression in placenta, brain neurons, and various cancers. For research use only.
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 0.5 µg/mL | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 | |
| ELISA | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from C-terminus of human GLUT3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2191309 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is placenta tissue.
Target Information
Glucose is fundamental to the metabolism of mammalian cells. Several glucose transporter protein (GLUT) isoforms have been identified and shown to function in response to insulin and IGF1 signaling.
GLUT3 is detectable in normal cell types such as spermatids in testis with active spermatogenesis, placental trophoblast membranes, and neurons in the brain.
GLUT3 staining is also detectable in human cancers including ovary, lung, and testis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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