Thermo Fisher Scientific GRP78 Polyclonal Antibody
GRP78 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. 인간 및 마우스 반응성, 항원 친화 크로마토그래피로 정제됨. Western blot, IHC, ICC 등 다양한 응용에 적합. 세포 항상성 및 단백질 접힘 연구에 유용.
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Thermo Fisher Scientific GRP78 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | View publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100 | View publication |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to near N-terminus of human GRP78 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.053 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2119999 |
Product Specific Information
This antibody is predicted to react with bovine, chicken, canine, amphibian, mouse, and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is breast carcinoma.
Target Information
GRP78 is a 78 kDa glucose-regulated protein belonging to the heat shock protein family (HSP70). It resides in the endoplasmic reticulum (ER) and transiently associates with newly synthesized secretory and membrane proteins.
It interacts with mutant and misfolded proteins, marking them for degradation. The conserved Lys-Asp-Glu-Leu (KDEL) sequence at its C-terminus ensures ER retention via the KDEL receptor.
GRP78 is essential for protein folding and degradation, maintaining cell homeostasis, and preventing apoptosis.
It serves as a biomarker for hypoglycemia and has neuroprotective roles under oxidative stress. Reduced GRP78 levels are observed in Alzheimer’s disease and presenilin-1 mutations.
Induction of GRP78 has been associated with drug resistance to antitumor agents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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