Thermo Fisher Scientific CCL27 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
FLLPPSTACC TQLYRKPLSD KLLRKVIQVE LQEADGDCHL QAFVLHLAQR SICIHPQNPS LSQWFEHQER KLHGTLPKLN FGMLRKMG

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human CTACK (CCL27). Anti-Human CTACK (CCL27)-specific antibody was purified by affinity chromatography and biotinylated.

Sandwich ELISA:
To detect Human CTACK by sandwich ELISA (100 µL/well), use 0.25–1.0 µg/mL of this antibody. When used with PeproTech Polyclonal Anti-Human CTACK (500-P294) as a capture antibody, it allows detection of at least 0.2–0.4 ng/well of Recombinant Human CTACK.

Western Blot:
To detect Human CTACK by Western Blot, use 0.1–0.2 mg/mL. Detection limit for Recombinant Human CTACK is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Package Information:
500-P294BT-1MG supplied as 2 × 500 µg.

Target Information

The CCL27 gene is part of the CC cytokine gene cluster on chromosome 9p. Cytokines are secreted proteins involved in immunoregulatory and inflammatory processes. CC cytokines are characterized by two adjacent cysteines. The protein encoded by this gene is chemotactic for skin-associated memory T lymphocytes and may mediate lymphocyte homing to cutaneous sites. It specifically binds to chemokine receptor 10 (CCR10). Studies of the murine homolog suggest a role in T cell–mediated skin inflammation.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.