Thermo Fisher Scientific CCL27 Polyclonal Antibody, PeproTech
인간 CCL27 단백질을 인식하는 토끼 폴리클로날 항체로, Western blot 및 ELISA에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공됩니다. 피부 관련 T세포 연구 및 염증 반응 연구에 유용합니다.
- 카탈로그번호
- 500-P294-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CCL27 Polyclonal Antibody, PeproTech
Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human CTACK (CCL27) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930265 |
Product Specific Information
AA Sequence of recombinant protein:
FLLPPSTACC TQLYRKPLSD KLLRKVIQVE LQEADGDCHL QAFVLHLAQR SICIHPQNPS LSQWFEHQER KLHGTLPKLN FGMLRKMG.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human CTACK (CCL27). Anti-Human CTACK (CCL27)-specific antibody was purified by affinity chromatography employing an immobilized Human CTACK (CCL27) matrix.
Sandwich ELISA:
To detect Human CTACK (CCL27) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human CTACK (CCL27) (500-P294Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human CTACK (CCL27).
Western Blot:
To detect Human CTACK by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human CTACK is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
The CCL27 gene is part of the CC cytokine gene cluster on chromosome 9p. Cytokines are secreted proteins involved in immunoregulatory and inflammatory processes. CC cytokines are characterized by two adjacent cysteines. The protein encoded by this gene is chemotactic for skin-associated memory T lymphocytes and may mediate lymphocyte homing to cutaneous sites. It specifically binds to chemokine receptor 10 (CCR10). Studies of murine homologs suggest a key role in T cell–mediated skin inflammation.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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