Thermo Fisher Scientific MyoD1 (Rhabdomyosarcoma Marker) Monoclonal Antibody (SPM427)
MyoD1 단백질을 인식하는 단클론 항체로, 횡문근육종(Rhabdomyosarcoma) 마커로 사용됩니다. IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합하며, Mouse IgG1 형식의 비결합 항체입니다. 단백질 A/G로 정제되어 고순도이며, -20°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM427 |
| Immunogen | Recombinant MyoD of Mouse origin with an epitope mapping to amino acids 180–189 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
Target Information
The Myogenic determination gene (MyoD) was first identified by its ability to convert embryonic mouse fibroblast cells into muscle cells. Forced expression of the MyoD gene (human homolog: myf3) can induce muscle-specific gene activation in various cell types. The regulatory domain of MyoD includes a 70 amino acid region containing a basic DNA-binding motif and a helix-loop-helix (HLH) dimerization motif. Related genes with homology to this domain include myf5, myogenin (myf4), and myf6 (MRF4, herculin).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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