Thermo Fisher Scientific MyoD1 (Rhabdomyosarcoma Marker) Monoclonal Antibody (SPM427)
MyoD1 단클론 항체(SPM427)는 Rhabdomyosarcoma 표지자로 사용되며, 인간·마우스·랫 등 다양한 종에 반응합니다. IHC, ICC/IF, Flow Cytometry에 적용 가능하며, 단백질 A/G로 정제된 액상 형태입니다. PBS 버퍼에 저장되며 4°C 보관을 권장합니다.
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Thermo Fisher Scientific MyoD1 (Rhabdomyosarcoma Marker) Monoclonal Antibody (SPM427)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM427 |
| Immunogen | Recombinant MyoD of Mouse origin, epitope mapping to amino acids 180–189 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The Myogenic determination gene (MyoD) was first identified for its ability to convert embryonic mouse fibroblast cells into muscle cells. Forced expression of MyoD (human homolog: myf3) in various normal and neoplastic cells can induce muscle cell conversion or activate muscle-specific genes that are otherwise transcriptionally inactive.
The regulatory domain of MyoD includes a 70 amino acid region with a basic DNA-binding motif and a helix-loop-helix (HLH) dimerization motif.
Related genes sharing sequence homology in the HLH domain include myf5, myogenin (myf4), and myf6 (MRF4/herculin).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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