Thermo Fisher Scientific CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), NovaFluor Yellow 700, eBioscience

Thermo Fisher Scientific CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), NovaFluor Yellow 700, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.8 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 737
• Brilliant Violet 650
• eFluor 450
• FITC
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The eBio124-1D1 monoclonal antibody reacts with human CD7 (gp40, Leu9), a 40 kDa receptor belonging to the immunoglobulin superfamily.
CD7 is expressed on most immature and mature T lymphocytes, T cell leukemias, natural killer cells, and a subset of normal and malignant B cells.
Cross-linking CD7 modulates T cell and NK cell activity, influencing calcium flux, adhesion molecule expression, cytokine secretion, and proliferation.
CD7 associates directly with phosphoinositol 3'-kinase, inducing D-3 phosphoinositide production and tyrosine phosphorylation.

A clonogenic CD34(+) CD38(-) cord blood cell population expressing CD45RA, HLA-DR, and high CD7 levels supports lymphopoiesis, generating B cells, NK cells, and dendritic cells but not myeloid or erythroid cells.
CD7(+) phenotype distinguishes primitive lymphoid progenitors from pluripotent stem cells.
Additionally, CD7 may cooperate with CD28 in Treg function; deficiency in both reduces Treg numbers and suppressive activity.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for flow cytometry of human peripheral blood cells at 5 µL (0.8 µg)/test in 100 µL final volume.
Cell count per test: 10⁵–10⁸ cells (empirically determined).

Note:
NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for optimal results.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01):
Required for optimal NovaFluor staining. Add 5 µL per sample before mixing with cells.
For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures minimizing spillover and spread.

Excitation: 552 nm
Emission: 700 nm
Laser: 561 nm (Yellow)

Target Information

CD7 (gp40, Leu9) is a 40 kDa member of the immunoglobulin gene superfamily.
The N-terminal (aa 1–107) is homologous to Ig kappa light chains; the C-terminal domain is proline-rich and forms a stalk structure.
CD7 is expressed on immature/mature T lymphocytes, T cell leukemia, NK cells, and some B cells.
Cross-linking CD7 modulates T and NK cell activity through calcium flux, adhesion, cytokine secretion, and proliferation.
CD7 directly associates with PI3-kinase, inducing D-3 phosphoinositides and tyrosine phosphorylation.
Expression of CD7 serves as a key marker in leukemia diagnostics.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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