Thermo Fisher Scientific CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), NovaFluor Yellow 610, eBioscience

Thermo Fisher Scientific CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), NovaFluor Yellow 610

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.8 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 737
• Brilliant Violet 650
• eFluor 450
• FITC
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 700
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The eBio124-1D1 monoclonal antibody reacts with human CD7 (gp40, Leu9), a 40 kDa receptor belonging to the immunoglobulin gene superfamily.
CD7 is expressed on most immature and mature T lymphocytes and T cell leukemias, as well as on NK cells, a subset of B cells, and malignant B cells.

Cross-linking of CD7 modulates T cell and NK cell activity, influencing calcium flux, adhesion molecule expression, cytokine secretion, and proliferation. CD7 associates directly with phosphoinositol 3'-kinase, and its ligation induces D-3 phosphoinositide production and tyrosine phosphorylation.

A clonogenic subpopulation of CD34(+) CD38(-) cord blood cells expressing CD45RA, HLA-DR, and high CD7 levels has lymphopoietic capacity, generating B cells, NK cells, and dendritic cells but not myeloid or erythroid cells. The CD7(+) phenotype marks primitive lymphoid progenitors.

CD7 may also cooperate with CD28 during Treg function; deficiency in both reduces Treg number and suppressive activity.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for use at 5 µL (0.8 µg) per test (100 µL final volume).
Cell numbers may vary (10⁵–10⁸ cells/test).

NovaFluor Compatibility:
Not compatible with DNA intercalating viability dyes (propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability testing.

CellBlox Plus Blocking Buffer:
Required for optimal staining with NovaFluor conjugates.
Add 5 µL per sample before combining with cells.
For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor Technology:
Based on Phiton™ nucleic acid dye structures engineered for optimized fluorescence and minimized spillover.
Excitation: 552 nm; Emission: 612 nm; Laser: 561 nm (Yellow).

Target Information

CD7 (gp40, Leu9) is a 40 kDa immunoglobulin superfamily member expressed on T lymphocytes, T cell leukemias, NK cells, and subsets of B cells.
It modulates cellular activation and signaling and serves as a key marker in leukemia diagnostics.

For Research Use Only. Not for use in diagnostic procedures.

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