Thermo Fisher Scientific CD47 Monoclonal Antibody (2D3), NovaFluor Blue 690, eBioscience

Thermo Fisher Scientific CD47 Monoclonal Antibody (2D3), NovaFluor Blue 690, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test
Publications: -

Product Specifications

Additional Formats

• Unconjugated
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• Super Bright 436
• Request custom conjugation available

Product Specific Information

The monoclonal antibody 2D3 reacts to CD47 (integrin-associated protein, IAP, neurophilin). CD47 is a glycosylated five-transmembrane protein involved in adhesion through interactions with SIRP and integrins CD51/CD61 and CD41/CD61. It mediates bi-directional signaling affecting neural synaptic activity and macrophage phagocytosis. CD47 also acts as a receptor for thrombospondin and is expressed in hematopoietic and non-hematopoietic cells.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for use at 5 µL (0.6 µg) per test with human peripheral blood cells.
A test is defined as staining a cell sample (10⁵–10⁸ cells) in a final volume of 100 µL.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) must be used for optimal staining. Add 5 µL per sample before combining with cells.

NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures designed to minimize spillover and spread.

Excitation: 494 nm
Emission: 585 nm
Laser: 488 nm (Blue)

Target Information

CD47 (integrin-associated protein, IAP) is a cell surface transmembrane glycoprotein interacting with integrins and regulating their functions. It modulates signaling pathways, intracellular calcium levels, and acts as a receptor for thrombospondin. CD47 interacts with CD172a (SIRPα) on macrophages to prevent phagocytosis of CD47-positive cells. It influences cell migration, adhesion, T cell activation, chondrocyte mechanotransduction, and neuronal development, especially in synapse-rich brain and retinal regions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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