Thermo Fisher Scientific CD47 Monoclonal Antibody (2D3), NovaFluor Blue 660-120S, eBioscience

Thermo Fisher Scientific CD47 Monoclonal Antibody (2D3), NovaFluor Blue 660-120S

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• Super Bright 436
• Request custom conjugation available

Product Specific Information

The monoclonal antibody 2D3 reacts with CD47, also known as integrin-associated protein (IAP) and neurophilin. CD47 is a glycosylated five-transmembrane protein involved in adhesion through interactions with SIRP (signal regulatory protein) and is associated with β3 integrins (CD51/CD61 and CD41/CD61). It mediates bi-directional signaling influencing neural synaptic activity and macrophage phagocytosis. CD47 also acts as a receptor for thrombospondin.

CD47 is expressed in most hematopoietic cells (T, B cells, monocytes, platelets, erythrocytes) and non-hematopoietic cells.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells at 5 µL (0.6 µg) per test.
A test corresponds to 100 µL final staining volume for 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell Stains for viability assays.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining with NovaFluor conjugates.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls: 5 µL per 100 µL of cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates utilize Phiton™ technology, based on novel nucleic acid dye structures engineered for optimized fluorescence with minimal spillover and spread.

Optical Properties:

• Excitation: 509 nm
• Emission: 665 nm
• Laser: 488 nm (Blue)

Target Information

CD47 (Integrin-Associated Protein, IAP) is a ubiquitously expressed transmembrane glycoprotein that interacts with integrins and modulates various signaling pathways, including G protein activation and calcium signaling. It serves as a receptor for the C-terminal domain of thrombospondin, influencing membrane transport, cell adhesion, and signal transduction.
CD47 binds CD172a (SIRPα) on macrophages to inhibit phagocytosis of CD47-positive cells and regulates immune responses, cell migration, and neuronal development.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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