Thermo Fisher Scientific CD47 Monoclonal Antibody (2D3), NovaFluor Red 710, eBioscience

Applications

• Tested Application: Flow Cytometry (Flow)
• Recommended Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• Super Bright 436
• Custom conjugation available

Product Specific Information

The monoclonal antibody 2D3 reacts with CD47, also known as integrin-associated protein (IAP) and neurophilin.
CD47 is a glycosylated five-transmembrane protein involved in adhesion via interactions with SIRP (signal regulator protein) and integrins (CD51/CD61, CD41/CD61). It mediates bi-directional signaling that affects neural synaptic activity and macrophage phagocytosis. CD47 also functions as a receptor for thrombospondin.

CD47 is expressed in most hematopoietic cells (T, B cells, monocytes, platelets, erythrocytes) and non-hematopoietic tissues.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications

• Reported for use in flow cytometric analysis
• Pre-titrated and tested on normal human peripheral blood cells
• Recommended use: 5 µL (0.6 µg) per test
• A test = staining of cell sample in 100 µL final volume (10⁵–10⁸ cells/test)

Important Notes

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for compatibility.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls: add 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor Technology

NovaFluor conjugates utilize Phiton™ technology, incorporating nucleic acid dye structures engineered for optimized fluorescent signatures with minimal spillover and spread.

• Excitation: 639 nm
• Emission: 710 nm
• Laser: 633–640 nm (Red)

Target Information

CD47 (Integrin-associated protein, IAP) is a ubiquitous transmembrane glycoprotein that interacts with integrins and regulates signaling pathways, including G protein activation and calcium mobilization upon cell adhesion.
It serves as a receptor for thrombospondin and modulates membrane transport, signal transduction, and immune functions.
CD47 acts as a ligand for CD172a (SIRPα), preventing phagocytosis of CD47-positive cells and influencing cell migration, B cell adhesion, T cell activation, and neuronal development.

For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.