Thermo Fisher Scientific Phospho-ERK1/2 (P44-MAPK) (Thr202, Tyr204) Polyclonal Antibody
ERK1/2 단백질의 Thr202 및 Tyr204 인산화 부위를 인식하는 폴리클로날 항체로, WB 및 IHC 분석에 적합합니다. 다양한 종에서 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. 세포 내 핵 위치 탐지에 활용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide derived from epitope -LTEYV- with the dual phosphorylation sites Thr202 and Tyr204 of ERK1/2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 25 mM Tris with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Kidney and spleen lysates
- Cellular location: Nucleus
Target Information
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells.
Various stimuli, including growth factors, cytokines, virus infection, ligands for G protein-coupled receptors, and transforming agents, activate the ERK1/2 pathways.
Upon receptor tyrosine kinase activation, Ras interacts with Raf, which phosphorylates MEK1/2. MEK1/2 subsequently phosphorylates ERK1/2 on tyrosine/threonine residues.
Activated ERKs phosphorylate cytoplasmic targets or translocate to the nucleus to activate transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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