Thermo Fisher Scientific ERK1/2 (P44-MAPK) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 0.1–1 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 2–10 µg/mL

Product Specifications

Product Specific Information

• Positive control: Kidney and spleen lysates
• Cellular location: Nucleus

Target Information

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells.
Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors, and transforming agents, activate the ERK1 and ERK2 pathways.
When growth factors bind to receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase, and activates it. Once activated, Raf phosphorylates serine residues in MEK1/2, which in turn phosphorylates tyrosine/threonine residues in ERK1/2.
Upon activation, ERKs phosphorylate cytoplasmic targets or migrate to the nucleus, where they activate transcription factors such as c-Fos and Elk-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.