Thermo Fisher Scientific ERK1/2 (P44-MAPK) Polyclonal Antibody
ERK1/2 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot 및 IHC(P) 실험에 적합하며, 다양한 종에 반응. 고순도 항원 친화 크로마토그래피로 정제되어 안정적이며 연구용으로 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 0.1–1 µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 2–10 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Chicken, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide derived from epitope -LTEYV- without the dual phosphorylation sites Thr202 and Tyr204 of ERK1/2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Kidney and spleen lysates
- Cellular location: Nucleus
Target Information
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells.
Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors, and transforming agents, activate the ERK1 and ERK2 pathways.
When growth factors bind to receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase, and activates it. Once activated, Raf phosphorylates serine residues in MEK1/2, which in turn phosphorylates tyrosine/threonine residues in ERK1/2.
Upon activation, ERKs phosphorylate cytoplasmic targets or migrate to the nucleus, where they activate transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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