Thermo Fisher Scientific TSLP Receptor Monoclonal Antibody (eBio1A6 (1A6)), PE, eBioscience

Thermo Fisher Scientific TSLP Receptor Monoclonal Antibody (eBio1A6 (1A6)), PE, eBioscience

Applications and Tested Dilution

• Western Blot (WB): Not tested
• Flow Cytometry (Flow): 5 µL (0.06 µg)/test

Publications

• Western Blot: View 1 publication
• Flow Cytometry: View 4 publications

Product Specifications

Available Formats

• Unconjugated
• APC
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

The eBio1A6 monoclonal antibody reacts with human thymic stromal-derived lymphopoietin receptor (TSLPR), a ~50 kDa protein similar to the common gamma-chain. TSLPR complexes with IL-7Rα (CD127) to form a high-affinity receptor for TSLP.
Human TSLPR is expressed by monocytes and CD11c⁺ dendritic cells. TSLP binding induces Th2 cell-attracting chemokines CCL17 and CCL22, and promotes secretion of Th cytokines IL-4, IL-5, and IL-13.
In mice, TSLPR deficiency alone shows no lymphocyte reduction, but double deficiency with the common gamma-chain reduces lymphocytes further.
The eBio1A6 antibody can cross-block binding of another anti-human TSLPR antibody, 1D8.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for flow cytometry using human TSLPR-transfected Ba/F3 cells. Recommended use: 5 µL (0.06 µg)/test in 100 µL final volume (10⁵–10⁸ cells/test).
Excitation: 488–561 nm
Emission: 578 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Thymic stromal lymphopoietin (TSLP) is a key cytokine that drives dendritic cell maturation and activation. It supports regulatory T cell selection, CD4⁺ T cell homeostasis, and allergic Th2 responses.
TSLP also promotes B cell progenitor growth and differentiation to IgM⁺ stage.
Its receptor, TSLP-R, binds TSLP with low affinity alone, but high affinity when co-expressed with IL-7Rα, forming a functional heteromeric complex.
Signal transduction requires STAT5 and is inhibited by SOCS-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지