Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), PE, eBioscience

Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), PE, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 660
• Alexa Fluor 700
• Alexa Fluor Plus 594
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506, 570, 615, 660
• FITC
• PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Custom conjugation available upon request

Product Specific Information

The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein expressed during all active phases of the cell cycle (G1, S, G2, and M), but absent from resting cells (G0). Ki-67 redistributes from the nucleus to chromosomes during mitosis and serves as a marker for cell proliferation.

SolA15 also recognizes human, non-human primate, and canine Ki-67.
Reported applications include flow cytometric analysis and intracellular staining.

Tested Application:
Intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Buffer Set (Product #00-5523-00). Recommended use ≤0.06 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Antibody titration is recommended for optimal results.

Excitation/Emission:
488–561 nm / 578 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases of the cell cycle. It associates with euchromatin, centromeric heterochromatin, and the nucleolus. Ki-67 expression varies topographically across cell cycle phases and is absent in G0 cells, making it a reliable proliferation marker. Ki-67 antibodies are commonly used to determine cell growth fractions in neoplasms and correlate strongly with tumor grade and prognosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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