Thermo Fisher Scientific PKC epsilon Recombinant Rabbit Monoclonal Antibody (JA38-21)
PKC epsilon 단백질을 인식하는 재조합 토끼 단일클론 항체로, 높은 특이성과 민감도를 제공합니다. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합합니다. 로트 간 일관성과 동물 유래 성분이 없는 포뮬레이션을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JA38-21 |
| Immunogen | Synthetic peptide within Human PKC epsilon (aa 561–610) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809992 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select optimal candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
The PKC family of serine/threonine kinases, including PRKCE (PKC epsilon), is activated intracellularly by signal transduction pathways. In humans, at least 12 PKC isoforms have been identified, differing in structure, localization, and substrate specificity.
Conventional PKC isoforms (alpha, beta I, beta II, gamma) are Ca²⁺- and phospholipid-dependent. PKC enzymes phosphorylate diverse protein targets and participate in multiple signaling pathways. They also act as major receptors for phorbol esters, known tumor promoters. Each PKC isoform exhibits distinct expression and cellular roles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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