Thermo Fisher Scientific ATP4B Monoclonal Antibody (2G11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:4,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

-

View 37 publications 37 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:2,000

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

1:2,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 11 publications 11 publications

Flow Cytometry (Flow)

1/50

View 3 publications 3 publications

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

ChIP assay (ChIP)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Dog, Human, Mustelid, Mouse, Pig, Rabbit, Rat

Published species

Bovine, Guinea pig, Human, Mouse, Rabbit, Rat, Rodent

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2G11

Immunogen

Purified 34 kDa core peptide from deglycosylated hog gastric microsomes. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ATP4B,uniProtId:P51164-1,ncbiNodeId:9606,antigenRange:1-291,antigenLength:291,antigenImageFileName:MA3-923_ATP4B_P51164-1_House_mouse.svg,antigenImageFileNamePDP:MA3-923_ATP4B_P51164-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

PBS, ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2227726

Product Specific Information

MA3-923 detects the beta-subunit of hydrogen/potassium ATPase from bovine, human, canine, porcine, rabbit, mouse, ferret, and rat tissues.

MA3-923 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, and immunoprecipitation procedures (ChIP). By Western blot, this antibody detects various forms of the beta-subunit, including a 60 to 80 kDa glycosylated protein, a 52 kDa beta-subunit precursor, and the 34 kDa core peptide from rabbit stomach extracts. Immunohistochemical staining of hydrogen/potassium ATPase in rabbit gastric glands with MA3-923 results in specific staining of parietal cells.

MA3-923 has been shown to inhibit the enzymatic activity of the hydrogen/potassium ATPase and to alter the affinity of the cytoplasmic potassium binding site.

The MA3-923 antigen is purified 34 kDa core peptide from deglycosylated hog gastric microsomes. This antibody recognizes an epitope between amino acid residues 1-13 or 15-28 located on the cytoplasmic side of the beta-subunit.

Target Information

The hydrogen/potassium ATPase, or gastric proton pump, belongs to a family of P-type cation-transporting ATPases. This family of ATPases shares a number of functional and structural similarities including the common feature of consisting of an alpha and beta subunit. Like the ubiquitous sodium/potassium ATPase, the hydrogen/potassium ATPase consists of a large transmembrane catalytic subunit, termed the alpha- subunit which contains sites for ATP binding and phosphorylation, and an associated smaller glycoprotein, termed the beta-subunit which may play a role in maintaining the structural and functional integrity of the complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.