Thermo Fisher Scientific Sodium/Calcium Exchanger Monoclonal Antibody (C2C12)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 67 publications 67 publications

Immunohistochemistry (IHC)

-

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1/100

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:200

View 12 publications 12 publications

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

in situ PLA (PLA)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Guinea pig, Human, Mouse, Rabbit, Rat

Published species

Bovine, Dog, Guinea pig, Human, Marsupial, Mouse, Pig, Rabbit, Rat, Rodent

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

C2C12

Immunogen

Purified canine cardiac sodium/calcium exchanger.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography - MBP

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2191111

Product Specific Information

MA3-926 detects sodium/calcium exchanger from human, canine, rabbit, mouse, guinea pig and rat kidney and cardiac tissues.

MA3-926 has been successfully used in Western blot, immunofluorescence, immunohistochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects a 120 kDa protein representing the sodium/calcium exchanger from guinea pig cardiac extract. The bands seen at 70 kDa and 160 kDa represent a proteolytic fragment and non-reduced exchanger respectively. MA3-926 is not recommended for Western blot procedures of rat tissues. Immunohistochemical staining of the sodium/calcium exchanger in rat heart with MA3-926 results in staining of the plasma membrane and intense staining of cardiac T-tubular membrane.

The MA3-926 antigen is purified canine cardiac sodium/calcium exchanger. This antibody recognizes an epitope between amino acids 371-525, which is on the intracellular side of the plasma membrane.

Target Information

Ca2+ plays a critical role in intracellular signaling and thus regulation of calcium balance is critical for the maintenance of this signaling function. The plasma membrane Na+/Ca2+ (NaCX) exchangers mediate Na+-dependent Ca2+ efflux in a wide variety of cell types. NaCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane. In mammals, at least 5 distinct genes code for the exchangers: Three NaCX (NaCX-1, NaCX-2, and NaCX-3), and two in the NaCKX family (NaCKX-1 and NaCKX-2). NaCX-1 is most prominently expressed in the heart where it plays a major role in excitation-contraction coupling (Matsuoka et al., 1993). NACX-1 may also be a target for protection of the heart and brain from ischemia and reperfusion injury (Hagihara et al., 2005; Bano et al., 2005; Choi, 2005).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.