Thermo Fisher Scientific RyR1 Monoclonal Antibody (34C)

Applications and Tested Dilution

Product Specifications

Product Specific Information

MA3-925 detects ryanodine receptor (RyR)-1 and RyR-2 isoforms in amphibian, chicken, bovine, canine, fish, human, non-human primate, mouse, rabbit, mink, rat, and sheep tissues.
In chickens, this antibody detects the alpha, beta, and cardiac isoforms. It also detects RyR-3 in mouse cells.
In frog, MA3-925 detects the alpha and beta isoforms, and in fish, the alpha isoform.

MA3-925 has been successfully used in Western blot, immunohistochemistry, immunofluorescence, and immunoprecipitation procedures.
By Western blot, this antibody detects a 565 kDa protein representing RyR from rat skeletal muscle extracts.
In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms.
Immunohistochemical staining of RyR in chicken brain with MA3-925 results in intense staining of cerebellar Purkinje neurons.

The MA3-925 antigen is partially purified chicken pectoral muscle ryanodine receptor.

Target Information

Ryanodine receptors play critical roles in intracellular Ca²⁺ signaling during cell activation in muscle and non-muscle cells.
Ryanodine receptor (~500 kDa) can be isolated from various cell types such as lymphocytes, macrophages, granulocytes, fibroblasts, epithelial and endothelial cells, skeletal and cardiac muscle, and brain tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.