Thermo Fisher Scientific RyR1 Monoclonal Antibody (34C)
Ryanodine 수용체(RyR1, RyR2, RyR3) 검출용 마우스 단클론 항체. Western blot, IHC, ICC/IF, IP 등 다양한 응용 가능. 여러 종(인간, 생쥐, 조류 등)에 반응. 고순도 Protein A 정제, -20°C 보관.
- 카탈로그번호
- MA3925
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:5,000 | View 98 publications |
| Immunohistochemistry (IHC) | - | View 16 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20 | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:1,000 | View 1 publication |
| Immunohistochemistry - Free Floating (IHC (Free)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 23 publications |
| Immunoprecipitation (IP) | Assay-dependent | View 9 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Amphibian, Bovine, Dog, Chicken, Fish, Human, Mustelid, Mouse, Non-human primate, Sheep, Rabbit, Rat |
| Published Species | Bovine, Cat, Chicken, Dog, Human, Mouse, Pig, Rabbit, Rat, Sheep, Zebrafish |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 34C |
| Immunogen | Partially purified chicken pectoral muscle ryanodine receptor |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2254138 |
Product Specific Information
MA3-925 detects ryanodine receptor (RyR)-1 and RyR-2 isoforms in amphibian, chicken, bovine, canine, fish, human, non-human primate, mouse, rabbit, mink, rat, and sheep tissues.
In chickens, this antibody detects the alpha, beta, and cardiac isoforms. It also detects RyR-3 in mouse cells.
In frog, MA3-925 detects the alpha and beta isoforms, and in fish, the alpha isoform.
MA3-925 has been successfully used in Western blot, immunohistochemistry, immunofluorescence, and immunoprecipitation procedures.
By Western blot, this antibody detects a 565 kDa protein representing RyR from rat skeletal muscle extracts.
In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms.
Immunohistochemical staining of RyR in chicken brain with MA3-925 results in intense staining of cerebellar Purkinje neurons.
The MA3-925 antigen is partially purified chicken pectoral muscle ryanodine receptor.
Target Information
Ryanodine receptors play critical roles in intracellular Ca²⁺ signaling during cell activation in muscle and non-muscle cells.
Ryanodine receptor (~500 kDa) can be isolated from various cell types such as lymphocytes, macrophages, granulocytes, fibroblasts, epithelial and endothelial cells, skeletal and cardiac muscle, and brain tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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