Thermo Fisher Scientific RyR1 Monoclonal Antibody (34C)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

View 98 publications 98 publications

Immunohistochemistry (IHC)

-

View 16 publications 16 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:1,000

View 1 publication 1 publication

Immunohistochemistry - Free Floating (IHC (Free))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 23 publications 23 publications

Immunoprecipitation (IP)

Assay-dependent

View 9 publications 9 publications

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Amphibian, Bovine, Dog, Chicken, Fish, Human, Mustelid, Mouse, Non-human primate, Sheep, Rabbit, Rat

Published species

Bovine, Cat, Chicken, Dog, Human, Mouse, Pig, Rabbit, Rat, Sheep, Zebrafish

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

34C

Immunogen

Partially purified chicken pectoral muscle ryanodine receptor.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2254138

Product Specific Information

MA3-925 detects ryanodine receptor (RyR)-1 and RyR-2 isoforms in amphibian, chicken, bovine, canine, fish, human, non-human primate, mouse, rabbit, mink, rat and sheep tissues. In chickens this antibody detects the alpha, beta and cardiac isoforms. This antibody also detects RyR-3 in mouse cells. In frog, MA3-925 detects the alpha and beta isoforms and in fish it detects the alpha isoform.

MA3-925 has been successfully used in Western blot, immunohistochemistry, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a 565 kDa protein representing RyR from rat skeletal muscle extracts. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor. Immunohistochemical staining of RyR in chicken brain with MA3-925 results in intense staining of cerebellar Purkinje neurons.

The MA3-925 antigen is partially purified chicken pectoral muscle ryanodine receptor.

Target Information

Ryanodine receptors have been shown to play critical roles in the intracellular Ca2+ signaling occurring during cell activation in muscle cells and non-muscled cells. Ryanodine receptor, ~500 kDa, can be isolated from a variety of cell types such as lymphocytes, macrophages, granulocytes, fibroblasts, epithelial, endothelial cells, skeletal muscles, cardiac muscle and brain tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.