Thermo Fisher Scientific ATP1A1 Monoclonal Antibody (M7-PB-E9)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:5,000

View 16 publications 16 publications

Immunohistochemistry (IHC)

-

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 4 publications 4 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Immunomicroscopy (IM)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Sheep, Pig, Rat, Bovine, Dog, Chicken

Published species

Dog, Human, Mouse, Pig, Rabbit, Rat, Sheep

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

M7-PB-E9

Immunogen

Purified alpha sodium/potassium ATPase from sheep kidney. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ATP1A1,uniProtId:P04074-1,ncbiNodeId:9940,antigenRange:1-1021,antigenLength:1021,antigenImageFileName:MA3-928_ATP1A1_P04074-1_House_mouse.svg,antigenImageFileNamePDP:MA3-928_ATP1A1_P04074-1_House_mouse_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 488
• Alexa Fluor 555
• Alexa Fluor 647
• Request custom conjugation

Form

Liquid

Concentration

1.87 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2060976

Product Specific Information

MA3-928 detects sodium/potassium ATPase alpha from human, mouse, bovine, sheep, canine, chicken and porcine tissues. This antibody detects the alpha 3 isoform in rat only.

MA3-928 has been successfully used in Western blot, immunohistochemistry, immunoprecipitation, immunocytochemistry, immunofluorescence, and ELISA procedures. By Western blot, this antibody detects an ~110 kDa protein representing alpha sodium/potassium ATPase from MDCK cell extract. Immunohistochemical staining of alpha sodium/potassium ATPase in porcine heart with MA3-928 yields a pattern consistent with the plasma membrane localization.

The MA3-928 antigen is purified sheep kidney alpha sodium/potassium ATPase. This antibody recognizes an epitope between amino acid residues 646 and 652 of the sheep kidney alpha sodium/potassium ATPase.

Target Information

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.