Thermo Fisher Scientific CACNA2D1 Monoclonal Antibody (20A)
CACNA2D1 단백질을 인식하는 Thermo Fisher monoclonal antibody (clone 20A). 다양한 종에서 반응하며 WB, IHC, ICC/IF, Flow Cytometry 등에 적합. 비결합형 액상 형태로 제공되며 연구용으로 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | View 34 publications |
| Immunohistochemistry (IHC) | – | View 5 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 | – |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:500 | – |
| Immunocytochemistry (ICC/IF) | 1:250 | View 5 publications |
| Flow Cytometry (Flow) | 1:100–1:200 | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Guinea pig, Human, Mouse, Rabbit, Rat |
| Published Species | Fish, Human, Mouse, Pig, Rabbit, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 20A |
| Immunogen | Purified rabbit dihydropyridine receptor |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2069904 |
Product Specific Information
MA3-921 detects 1,4-dihydropyridine (DHP) receptor alpha-2 subunit from human, rat, mouse, guinea pig, and rabbit skeletal and cardiac muscle.
It has been successfully used in Western blot, immunocytochemistry, immunofluorescence, and immunohistochemistry procedures.
By Western blot, this antibody detects a 220 kDa protein under non-reducing and a 143 kDa protein under reducing conditions, representing DHP from rabbit skeletal muscle membrane preparations.
Immunohistochemical staining of DHP in rabbit skeletal muscle with MA3-921 results in double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines.
The MA3-921 antigen is purified rabbit DHP receptor.
Target Information
This gene encodes a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex.
Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio.
Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits.
Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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