Thermo Fisher Scientific CACNA2D1 Monoclonal Antibody (20A)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 34 publications 34 publications

Immunohistochemistry (IHC)

-

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500

-

Immunohistochemistry (Frozen) (IHC (F))

1:500

-

Immunocytochemistry (ICC/IF)

1:250

View 5 publications 5 publications

Flow Cytometry (Flow)

1:100-1:200

View 2 publications 2 publications

Product Specifications

Species Reactivity

Guinea pig, Human, Mouse, Rabbit, Rat

Published species

Fish, Human, Mouse, Pig, Rabbit, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

20A

Immunogen

Purified rabbit dihydropyridine receptor. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CACNA2D1,uniProtId:P13806-1,ncbiNodeId:9986,antigenRange:1-1106,antigenLength:1106,antigenImageFileName:MA3-921_CACNA2D1_P13806-1_House_mouse.svg,antigenImageFileNamePDP:MA3-921_CACNA2D1_P13806-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2069904

Product Specific Information

MA3-921 detects 1,4-dihydropyridine (DHP) receptor alpha-2 subunit from human, rat, mouse, guinea pig and rabbit skeletal and cardiac muscle.

MA3-921 has been successfully used in Western blot, immunocytochemistry, immunofluorescence, and immunohistochemistry procedures. By Western blot, this antibody detects a 220 kDa protein under non-reducing and a 143 kDa protein under reducing conditions representing DHP from rabbit skeletal muscle membrane preparations. Immunohistochemical staining of DHP in rabbit skeletal muscle with MA3-921 results in double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines.

The MA3-921 antigen is purified rabbit DHP receptor.

Target Information

This gene encodes a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.