Thermo Fisher Scientific ENO1 Recombinant Rabbit Monoclonal Antibody (JM63-53)
Thermo Fisher Scientific의 ENO1 Recombinant Rabbit Monoclonal Antibody (JM63-53)은 인간, 마우스, 랫, 제브라피시에 반응하는 재조합 단클론 항체로, 높은 특이성과 일관성을 제공합니다. Western blot, ICC/IF, Flow cytometry에 적합하며, Protein A 정제 및 TBS buffer로 안정화되어 있습니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Zebrafish |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM63-53 |
| Immunogen | Synthetic peptide within Human ENO1 (aa 1–50) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810033 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to large rabbit immune repertoire
Target Information
Enolase (2-phosphoglycerate hydrolyase or phosphopyruvate hydrates) is a glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. It consists of three subunits: alpha, beta, and gamma.
Non-neuronal Enolase (ENO1) is an isoform composed of two alpha subunits. The ENO1 gene also encodes tau-crystallin, a monomeric structural lens protein.
Biological Roles:
- Involved in glycolysis and energy metabolism
- Participates in growth control, hypoxia tolerance, and allergic responses
- Functions as a plasminogen receptor and activator on cell surfaces
- Used as a diagnostic marker for tumors, hypoxic brain injury, and endometriosis
- Identified as an autoantigen in Hashimoto encephalopathy (HE) and associated with Hashimoto thyroiditis (HT)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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