Thermo Fisher Scientific MCM5 Polyclonal Antibody
Thermo Fisher Scientific의 MCM5 Polyclonal Antibody는 인간, 마우스, 랫트 시료에 반응하는 Rabbit IgG 항체입니다. Western blot, IHC, ICC/IF, ELISA, IP 등 다양한 응용에 사용 가능하며, 핵 내 MCM5 단백질 검출에 적합합니다. 고순도 친화 크로마토그래피 정제 및 안정적인 PBS/glycerol 저장용액 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 1–230 of human MCM5 (NP_006730.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.75 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913792 |
Product Specific Information
- Positive test controls: Raji, HL-60, HeLa, SW620, Mouse kidney, Rat kidney
- Target localization: Nucleus
Immunogen sequence:
MSGFDDPGIF YSDSFGGDAQ ADEGQARKSQ LQRRFKEFLR QYRVGTDRTG FTFKYRDELK RHYNLGEYWI EVEMEDLASF DEDLADYLYK QPAEHLQLLE EAAKEVADEV TRPRPSGEEV LQDIQVMLKS DASPSSIRSL KSDMMSHLVK IPGIIIAASA VRAKATRISI QCRSCRNTLT NIAMRPGLEG YALPRKCNTD QAGRPKCPLD PYFIMPDKCK CVDFQTLKLQ
Target Information
The protein encoded by this gene is structurally similar to the CDC46 protein from S. cerevisiae, which is involved in DNA replication initiation. It belongs to the MCM family of chromatin-binding proteins and interacts with other family members. The protein is upregulated during the G0 to G1/S phase transition and may play a role in cell cycle regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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