Thermo Fisher Scientific Phospho-14-3-3 zeta (Ser58) Polyclonal Antibody
14-3-3 zeta 단백질의 Ser58 인산화 형태를 특이적으로 검출하는 Rabbit Polyclonal Antibody. WB, IHC, ICC/IF에 적합하며 고순도 액상 형태로 제공. 인산화 신호전달 연구 및 세포주기 조절 관련 연구에 유용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human YWHAZ (Accession P63104), corresponding to amino acid residues around phosphorylated Ser58 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816407 |
Product Specific Information
This antibody detects endogenous levels of 14-3-3 zeta only when phosphorylated at Serine 58.
Target Information
The 14-3-3 family consists of at least seven highly conserved isoforms expressed in all eukaryotic cells. These proteins function as regulators of signal transduction by binding to serine-phosphorylated proteins.
14-3-3 interacts with Cdc25C to regulate cell cycle entry and with Bad to inhibit apoptosis. Other known binding partners include protein kinase C family members, Cbl, IRS-1, polyoma middle-T antigen, nitrate reductase, S-raf, and the IGF-1 receptor.
Detection of 14-3-3 proteins in cerebrospinal fluid is useful in the differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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