Thermo Fisher Scientific Phospho-14-3-3 zeta (Thr232) Polyclonal Antibody
14-3-3 zeta 단백질의 Thr232 인산화 형태를 특이적으로 검출하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF에 사용 가능. 인간, 마우스, 랫트 반응성. 고순도 정제, PBS/glycerol buffer, -20°C 보관.
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Thermo Fisher Scientific Phospho-14-3-3 zeta (Thr232) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human YWHAZ (Accession P63104), corresponding to amino acid residues around phosphorylated Thr232 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816408 |
Product Specific Information
Antibody detects endogenous levels of 14-3-3 zeta/delta only when phosphorylated at Threonine 232.
Target Information
At least seven isoforms comprise the highly conserved 14-3-3 family of homo- and heterodimeric proteins that are abundantly expressed in all eukaryotic cells. Although more than seven isoforms of 14-3-3 have been described, some redundancies have appeared upon sequencing.
The 14-3-3 proteins are key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. By interacting with Cdc25C, 14-3-3 regulates entry into the cell cycle, and through interaction with Bad, prevents apoptosis.
Other proteins that have been shown to bind to 14-3-3s include members of the protein kinase C family, Cbl, IRS-1, polyoma middle-T antigen, nitrate reductase, S-raf, and the IGF-1 receptor.
Detection of 14-3-3 proteins in cerebrospinal fluid has been shown to be useful in the differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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