Thermo Fisher Scientific Phospho-LIMK1/LIMK2 (Thr508, Thr505) Polyclonal Antibody
인산화된 LIMK1/LIMK2 (Thr508/Thr505)을 특이적으로 인식하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF 등 다양한 응용 가능. 고순도 정제 및 안정적 액상 형태로 제공되며, 세포 신호전달 및 형태학 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human LIMK1 (Accession P53667), corresponding to amino acid residues around phosphorylated Thr508. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816397 |
Product Specific Information
Antibody detects endogenous levels of LIMK1/2 only when phosphorylated at Threonine 508/505.
Target Information
Proteins containing LIM motifs are typically involved in cell fate determination and growth control. A family of proteins designated LIM kinases, including LIMK-1 and LIMK-2, has been identified.
LIMK-1 regulates F-actin stabilization and cofilin activity, playing a role in signaling pathways related to cell motility and morphogenesis. It inhibits neuronal differentiation of PC12 cells, likely by interfering with events downstream of MAPK activation.
Expression patterns of LIMK-1 and LIMK-2 suggest differing functions during development. A truncated form of LIMK-2 found in adult testis may arise from alternative exon initiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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