Thermo Fisher Scientific nNOS Polyclonal Antibody
뇌형 질소산화효소(nNOS)를 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF 등 다양한 응용에 적합. eNOS 및 iNOS와 교차반응 없음. -20°C 보관, 연구용 전용.
- 카탈로그번호
- PA3032A
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific nNOS Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 6 publications |
| Immunohistochemistry (IHC) | - | View 7 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:400 | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:800 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:250 | View 2 publications |
| Immunoprecipitation (IP) | Assay-dependent | View 2 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rabbit, Rat |
| Published Species | Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues T(724) K R R A I G F K K L A E A V K(739) C of rat nNOS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Whole serum |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2236017 |
Product Specific Information
PA3-032A detects brain nitric oxide synthase (bNOS) from bovine, mouse, rabbit, and rat tissues.
This antibody does not detect endothelial NOS (eNOS) or inducible NOS (iNOS).
PA3-032A has been successfully used in Western blot, immunoprecipitation, and immunohistochemical procedures.
By Western blot, this antibody detects an ~155 kDa protein representing bNOS from rat brain extract.
Immunohistochemical staining of bNOS in rat brain with PA3-032A results in staining of the anterior pituitary.
The PA3-032A immunogen is a synthetic peptide corresponding to residues T(724) K R R A I G F K K L A E A V K(739) C of rat nNOS.
Target Information
NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH, and O₂) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme).
Several distinct NOS isoforms have been described and shown to represent the products of three distinct genes.
These include two constitutive Ca²⁺/CaM-dependent forms of NOS:
- NOS1 (ncNOS), first identified in neurons
- NOS3 (ecNOS), first identified in endothelial cells
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.