Thermo Fisher Scientific nNOS Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

PA3-032A detects brain nitric oxide synthase (bNOS) from bovine, mouse, rabbit, and rat tissues.
This antibody does not detect endothelial NOS (eNOS) or inducible NOS (iNOS).

PA3-032A has been successfully used in Western blot, immunoprecipitation, and immunohistochemical procedures.
By Western blot, this antibody detects an ~155 kDa protein representing bNOS from rat brain extract.
Immunohistochemical staining of bNOS in rat brain with PA3-032A results in staining of the anterior pituitary.

The PA3-032A immunogen is a synthetic peptide corresponding to residues T(724) K R R A I G F K K L A E A V K(739) C of rat nNOS.

Target Information

NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH, and O₂) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme).
Several distinct NOS isoforms have been described and shown to represent the products of three distinct genes.
These include two constitutive Ca²⁺/CaM-dependent forms of NOS:

• NOS1 (ncNOS), first identified in neurons
• NOS3 (ecNOS), first identified in endothelial cells

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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