Thermo Fisher Scientific CYP2C11 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,500

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

-

Immunohistochemistry (Frozen) (IHC (F))

1:100

-

Immunocytochemistry (ICC/IF)

1:20-1:200

-

ELISA (ELISA)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Cat, Human, Rat

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptides corresponding to residues D(49) I G Q S I K K F S K V(60) and Q(491) R A D S L S S H L(500) of rat Cytochrome P450 2C11. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CYP2C11,uniProtId:P08683-1,ncbiNodeId:10114,antigenRange:49-60|491-500,antigenLength:500,antigenImageFileName:PA3-034_CYP2C11_P08683-1_Rabbit.svg,antigenImageFileNamePDP:PA3-034_CYP2C11_P08683-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum, PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_325981

Product Specific Information

PA3-034 detects cytochrome P450 2C11 (P450 2C11) from human, feline and rat tissues.

PA3-034 has been successfully used in Western blot, ICC/IF and immunohistochemical (frozen and paraffin) procedures. By Western blot, this antibody detects an ~50 kDa protein representing P450 2C11 from rat liver extract. Immunohistochemical staining of P450 2C11 in rat brain with PA3-034 results in perivascular staining.

The PA3-034 immunogen is a synthetic peptides corresponding to residues D(49) I G Q S I K K F S K V(60) and Q(491) R A D S L S S H L(500) of rat Cytochrome P450 2C11.

Target Information

The cytochrome P450 (P450) family of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as NADPH and P450 reductase. There are over 200 cDNAs which encode P450 protein. Epoxygenases are those P450 proteins which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450 proteins which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). EETs, which exhibit vasodilation activity, are formed when an epoxide group is inserted between the unsaturated carbons of AA in positions 5,6; 8,9; 11,12; 14,15. EETs are produced in cerebral cortical tissue, coronary arteries and vascular endothelium. EETs are converted from AA by the 2C11 family of P450`s whose expression is induced by testosterone and is therefore not generally found in females.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.