Thermo Fisher Scientific eNOS Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

-

View 16 publications 16 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:250

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:250

View 4 publications 4 publications

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Dog, Human, Mouse, Rat

Published species

Bovine, Dog, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues P(599) Y N S S P R P E Q H K S Y K(613) C of bovine eNOS. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:eNOS,uniProtId:P29473-1,ncbiNodeId:9913,antigenRange:599-613,antigenLength:1205,antigenImageFileName:PA3-031A_eNOS_P29473-1_Rabbit.svg,antigenImageFileNamePDP:PA3-031A_eNOS_P29473-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum, PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2251447

Product Specific Information

PA3-031A detects endothelial Nitric Oxide Synthase (eNOS) from canine, bovine, human, mouse and rat tissues. This antibody does not detect brain NOS (bNOS) or inducible NOS (iNOS).

PA3-031A has been successfully used in Western blot, immunohistochemistry, immunofluorescence, and immunocytochemistry procedures. By Western blot, this antibody detects an approximately 140 kDa band representing eNOS from canine cardiac extracts. Immunohistochemical staining of eNOS in mouse cardiac tissue with PA3-031 results in the staining of the endothelium.

The PA3-031A immunogen is a synthetic peptide corresponding to residues P(599) Y N S S P R P E Q H K S Y K(613) C of bovine eNOS.

Target Information

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.