Thermo Fisher Scientific eNOS Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

PA3-031A detects endothelial Nitric Oxide Synthase (eNOS) from canine, bovine, human, mouse, and rat tissues. This antibody does not detect brain NOS (bNOS) or inducible NOS (iNOS).

It has been successfully used in Western blot, immunohistochemistry, immunofluorescence, and immunocytochemistry applications.
By Western blot, this antibody detects an approximately 140 kDa band representing eNOS from canine cardiac extracts.
Immunohistochemical staining of eNOS in mouse cardiac tissue with PA3-031 results in specific staining of the endothelium.

The immunogen is a synthetic peptide corresponding to residues P(599) Y N S S P R P E Q H K S Y K(613) C of bovine eNOS.

Target Information

Nitric oxide (NO) is an inorganic, gaseous free radical that mediates various intercellular signals, including vasorelaxation, neurotransmission, and cytotoxicity.
NO production is catalyzed by nitric oxide synthase (NOS), which oxidizes L-arginine to produce L-citrulline and NO.

There are three main NOS isoforms:

• b/nNOS (type I) – brain or neuronal NOS
• eNOS (type III) – endothelial cell NOS
• iNOS (type II) – inducible NOS

All isoforms contain calmodulin, NADPH, FAD, and FMN binding domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.