Thermo Fisher Scientific CHRM3 Polyclonal Antibody
CHRM3 단백질을 표적으로 하는 Thermo Fisher Scientific의 rabbit polyclonal antibody. 인간 및 비인간 영장류에 반응하며, IHC(P) 및 ELISA에 사용 가능. 항원 친화 크로마토그래피로 정제된 액상 형태로 제공되며 연구용으로 적합.
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Applications
Immunohistochemistry (Paraffin) [IHC(P)]
- Tested Dilution: 10 µg/mL
- 참고: Heat-induced antigen retrieval in pH 6.0 citrate buffer 권장
ELISA
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic 20 amino acid peptide from 3rd cytoplasmic domain of human CHRM3 / M3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2550172 |
Product Specific Information
Percent identity with other species by BLAST analysis:
- 100%: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset
- 95%: Mouse, Rat, Sheep, Bovine, Hamster, Elephant, Horse, Rabbit, Pig
- 90%: Dog, Opossum
- 85%: Lizard
- 80%: Panda, Guinea pig
For IHC(P), use heat-induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Target Information
The muscarinic cholinergic receptors belong to a family of G protein-coupled receptors. Their functional diversity is defined by acetylcholine binding, resulting in cellular responses such as:
- Adenylate cyclase inhibition
- Phosphoinositide degeneration
- Potassium channel mediation
Muscarinic receptors influence acetylcholine effects in the central and peripheral nervous systems. The muscarinic cholinergic receptor 3 (CHRM3) controls smooth muscle contraction and its stimulation causes secretion of glandular tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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