Thermo Fisher Scientific CHRM3 Polyclonal Antibody
Rabbit polyclonal antibody against human CHRM3 for IHC and ELISA applications. High species cross-reactivity confirmed by BLAST analysis. Antigen affinity purified, unconjugated liquid form. Suitable for research use only.
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- PA532708
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- pk
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Applications
Immunohistochemistry (Paraffin) [IHC (P)]
- Tested Dilution: 10 µg/mL
- Note: Use heat-induced antigen retrieval in pH 6.0 citrate buffer. After incubation with primary antibody, slides were treated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
ELISA
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic 18 amino acid peptide from 3rd cytoplasmic domain of human CHRM3 / M3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2550171 |
Product Specific Information
Percent identity with other species by BLAST analysis:
- 100%: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset
- 94%: Orangutan, Sheep, Bovine, Hamster, Horse
- 89%: Elephant, Pig
- 83%: Mouse, Rat, Dog, Rabbit, Opossum, Guinea pig, Turkey, Chicken, Lizard
Target Information
The muscarinic cholinergic receptors are G protein-coupled receptors that mediate acetylcholine-induced cellular responses including adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel modulation. Muscarinic receptor 3 (CHRM3) regulates smooth muscle contraction and stimulates glandular secretion, influencing both central and peripheral nervous system functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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