Thermo Fisher Scientific CD71 (Transferrin Receptor) Monoclonal Antibody (R17217 (RI7 217.1.4)), Biotin, eBioscience
Mouse CD71 (Transferrin Receptor) 인식하는 Rat 모노클로날 항체로 Biotin 결합형. Flow Cytometry에 최적화되어 있으며 세포 성장 및 철분 흡수 관련 연구에 유용. 0.25 µg/test 권장, 4°C 암소 보관.
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Thermo Fisher Scientific CD71 (Transferrin Receptor) Monoclonal Antibody (R17217 (RI7 217.1.4)), Biotin, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 20 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published Species | Fish, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | R17217 (RI7 217.1.4) |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466500 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- eFluor 450
- FITC
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Blue 690
- NovaFluor Red 685
- NovaFluor Red 710
- NovaFluor Red 755
- NovaFluor Yellow 690
- NovaFluor Yellow 730
- NovaFluor Yellow 755
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The R17217 monoclonal antibody reacts with mouse CD71, a 170–180 kDa type II transmembrane protein known as the transferrin receptor. CD71 exists as a homodimer on the cell surface and is essential for cellular growth. It is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates CD71 expression, with strain-dependent differences observed in mice.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes and bone marrow cells, recommended ≤ 0.25 µg/test (100 µL final volume). Optimal performance requires titration.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Cellular iron uptake occurs via receptor-mediated endocytosis of transferrin receptor complexes. Endosomal acidification releases iron, and the apotransferrin-receptor complex is recycled to the cell surface. The transferrin receptor is essential for erythrocyte and nervous system development. Serum transferrin receptor (sTfR) serves as a biomarker for detecting erythropoietin misuse and diagnosing various anemias, including those linked to rheumatoid arthritis, pregnancy, IBS, and HIV.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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