Thermo Fisher Scientific CD69 Monoclonal Antibody (FN50), Biotin, eBioscience

Thermo Fisher Scientific CD69 Monoclonal Antibody (FN50), Biotin, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 561
• Alexa Fluor 660
• APC
• APC-eFluor 780
• Brilliant UV 395
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 650
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• FITC
• NovaFluor Blue 610-70S
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation available

Product Specific Information

The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 (~30 kDa) is expressed on the cell surface as a disulfide-linked dimer and is rapidly upregulated upon activation. It is found on lymphocytes, monocytes, and platelets.

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Verified by flow cytometric analysis of resting and 6-hour TPA-activated human peripheral blood cells. Recommended use ≤ 0.5 µg/test (100 µL final volume). Cell number may vary from 10⁵–10⁸ cells/test; titration for optimal performance is advised.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide-bonded homodimer (~60 kDa under non-reducing conditions). It contains one or two N-linked oligosaccharides and is present on activated platelets. In normal peripheral blood, a variable percentage of cells express CD69, which is involved in lymphocyte signal transduction. Expression is induced upon T lymphocyte activation and may play a role in proliferation and signal transmission in NK cells and platelets. Alternative splicing leads to multiple transcript variants. CD69 dysfunction is associated with diseases such as coccidiodomycosis and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.