Thermo Fisher Scientific CD69 Monoclonal Antibody (H1.2F3), Biotin, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 496
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 786
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and expressed as a disulfide-linked dimer. A subset of lymphocytes in thymus, spleen, and lymph nodes express this antigen, and its expression is rapidly upregulated upon T and B cell activation, indicating its role in lymphocyte development and activation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of resting and activated mouse splenocytes. Recommended ≤0.5 µg per test (100 µL final volume, 10⁵–10⁸ cells/test). Titrate for optimal performance.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide-bonded homodimer (~60 kDa under non-reducing conditions). It contains one or two N-linked oligosaccharides and is present on activated platelets. In normal peripheral blood, CD69 expression varies and is involved in lymphocyte signal transduction. Expression is induced upon T cell activation and may play a role in proliferation and signal transmission in NK cells and platelets. Multiple transcript variants exist. CD69 dysfunction is associated with coccidioidomycosis and asthma.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.