Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES3-9D7), eFluor 450, eBioscience
인간 IL-10을 인식하는 JES3-9D7 클론의 Rat IgG1 단클론 항체. eFluor 450 형광체 결합으로 Flow Cytometry에 최적화. Violet laser(405 nm)로 여기, 445 nm 방출. Affinity chromatography 정제, 4°C 보관, 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Flow Cytometry (Flow) | 5 µL (1 µg)/test | View 9 publications |
| ELISA | - | View 4 publications |
| Functional Assay | - | View 1 publication |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Rhesus monkey |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | JES3-9D7 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10548941 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- Brilliant UV 395
- Brilliant UV 737
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 711
- eFluor 660
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
Description:
The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).
Applications Reported:
Used for intracellular staining followed by flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by intracellular staining and flow cytometric analysis of Th2 polarized human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00) and BestProtocols® Protocol A.
Recommended usage: 5 µL (1 µg) per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.
Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®.
- Excitation: 405 nm
- Emission: 445 nm
- Laser: Violet Laser
Ensure instrument compatibility.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
IL-10 encodes a cytokine mainly produced by monocytes and lymphocytes. It regulates immune responses and inflammation by suppressing Th1 cytokines, MHC class II, and costimulatory molecules on macrophages.
Enhances B cell survival, proliferation, and antibody production. Inhibits NF-kappa B activity and participates in JAK-STAT signaling regulation.
Knockout studies suggest IL-10 is essential for intestinal immune regulation. Mutations are associated with HIV-1 susceptibility and rheumatoid arthritis.
Diseases associated with IL10 include Graft-Versus-Host Disease and HIV-1 infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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