Thermo Fisher Scientific IL-1 beta (Pro-form) Monoclonal Antibody (NJTEN3), eFluor 450, eBioscience
Mouse IL-1 beta pro-form 특이 단일클론 항체로, 염증성 사이토카인 연구에 적합. eFluor 450 형광 표지로 Flow Cytometry에 최적화. 0.5 µg/test 권장, 405/445 nm Violet Laser 사용. 4°C 보관, 동결 금지.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 21 publications |
| ELISA | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), eFluor 450, eBioscience |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NJTEN3 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574108 |
Available Formats
Product Specific Information
This NJTEN3 monoclonal antibody reacts with the pro-form of mouse IL-1 beta, a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It recognizes only the pro-form and not the active (cleaved) form.
Pro-IL-1 beta (31 kDa) is synthesized in response to inflammatory stimuli and requires inflammasome activation for cleavage into the active 17 kDa form. IL-1 beta is secreted via a non-classical pathway and signals through IL-1RI, shared with IL-1 alpha. These cytokines play key roles in innate immunity and inflammation regulation.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested on stimulated mouse peritoneal macrophages using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00) and BestProtocols® Protocol A.
Use ≤0.5 µg/test (100 µL final volume). Adjust cell number empirically (10⁵–10⁸ cells/test). Titrate antibody for optimal performance.
Fluorochrome: eFluor® 450 (alternative to Pacific Blue®)
- Excitation: 405 nm
- Emission: 445 nm
- Laser: Violet Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Interleukin-1 beta (IL-1β) is a proinflammatory cytokine produced by monocytes, macrophages, and dendritic cells. It is synthesized as a 31 kDa pro-form and processed to an active 17 kDa form by caspase-1 after inflammasome activation. IL-1β signals through IL-1RI and IL-1RII (shared with IL-1α), promoting cytokine production and acute-phase responses. IL-1RA can inhibit IL-1β activity through receptor competition. Elevated IL-1β is linked to chronic inflammatory diseases, and IL-1β neutralization has therapeutic potential.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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