Thermo Fisher Scientific IL-9 Monoclonal Antibody (MH9D1), eFluor 450, eBioscience
IL-9 단백질을 인식하는 MH9D1 단클론 항체로, eFluor 450 형광체가 결합된 제품입니다. 인간 세포 내 염색 및 유세포 분석에 최적화되어 있으며, Th9 세포 연구에 활용됩니다. 405/445 nm 파장에서 검출 가능하며, 4°C에서 보관합니다.
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Thermo Fisher Scientific IL-9 Monoclonal Antibody (MH9D1), eFluor 450, eBioscience
Applications
- Intracellular staining followed by flow cytometric analysis
- Tested on Th9 polarized human peripheral blood cells
Tested Dilution
- 5 µL (0.125 µg)/test
Publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MH9D1 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574107 |
Additional Formats
Product Specific Information
The monoclonal antibody MH9D1 recognizes interleukin-9 (IL-9), a proinflammatory cytokine involved in type 2 immune responses. Recent studies indicate IL-9 is secreted by a distinct T-helper lineage called Th9, derived from Th2 cells with TGF beta or directly from naive CD4+ T cells with TGF beta and IL-4.
IL-9 belongs to the cytokine receptor gamma chain-dependent family (IL-2, IL-4, IL-7, IL-15, IL-21). It affects Th2 lymphocytes, B lymphocytes, mast cells, eosinophils, and epithelial cells, implicating IL-9 in asthma and allergy-related disorders.
Staining with MH9D1 is not blocked by MH9A4, indicating distinct epitopes.
Applications Reported: Intracellular staining and flow cytometric analysis.
Applications Tested: Pre-titrated and tested with Th9 polarized human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00) and BestProtocols® Protocol A.
A test is defined as the amount (µg) of antibody that stains a sample in 100 µL final volume. Cell numbers may range from 10⁵ to 10⁸ cells/test.
Fluorochrome Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emits at 445 nm. Compatible with Violet laser detection systems.
Filtered post-manufacture with 0.2 µm membrane.
Target Information
Interleukin 9 (IL-9) is produced by activated Th2 lymphocytes and regulates cell types involved in asthma responses, including B/T lymphocytes, mast cells, eosinophils, and epithelial cells.
Mouse IL-9 cDNA encodes a 144 amino acid precursor with an 18 amino acid signal peptide, forming a mature cysteine-rich protein (~14 kDa). Native mouse IL-9 is heavily glycosylated.
TGF beta influences differentiation of IL-17-producing T helper and regulatory T cells by reprogramming Th2 cells to secrete IL-9, driving Th9 cell formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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