Thermo Fisher Scientific SCF Polyclonal Antibody, PeproTech

Thermo Fisher Scientific SCF Polyclonal Antibody (PeproTech)

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MQEICRNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV THLSVSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVAC MEENAPKNVK ESLKKPETRN FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Rat SCF. Anti-Rat SCF-specific antibody was purified by affinity chromatography employing an immobilized Rat SCF matrix.

Sandwich ELISA:
To detect Rat SCF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat SCF (500-P202Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Rat SCF.

Western Blot:
To detect Rat SCF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant Rat SCF is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Target Information

Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization and activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase.
SCF expression induces mast cell survival and release of histamine, cytokines, and chemokines. Inhibition of the SCF/c-Kit pathway reduces histamine levels, mast cell and eosinophil infiltration, IL-4 production, and airway hyperresponsiveness, suggesting therapeutic potential in inflammatory diseases like asthma.
At least two isoforms of SCF are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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