Thermo Fisher Scientific Interleukin-1 alpha (IL-1 alpha) Monoclonal Antibody (IL1A, 3982)

Applications and Tested Dilution

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Peptide Array (Array)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It is encoded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both shared with IL-1 beta. The IL1A gene is located on chromosome 2q13.

IL-1 alpha plays a critical role in innate host defense by inducing other proinflammatory cytokines and initiating acute-phase responses. Its activity can be regulated by IL-1 Receptor Antagonist (IL-1RA), which blocks receptor binding through competitive inhibition.

This cytokine is involved in immune response, inflammation, and hematopoiesis. It is produced as a proprotein by monocytes and macrophages, processed upon cell injury, and induces apoptosis. IL-1 alpha regulates NF-κB and MAP kinase pathways, stimulates IL6 and COX-2 (PTGS2) expression, and enhances nitric oxide (NO) production. Elevated IL-1 alpha levels are associated with chronic inflammatory diseases such as rheumatoid arthritis, psoriasis, and multiple sclerosis.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.